The 23-year-old has returned to the Premier League club for what the Football Association says is “assessment of an ongoing issue”.
Manager Gareth Southgate has decided not to call up any replacements.
James missed last year’s World Cup with a knee injury and picked up another knee problem in December which kept him out for another month.
The remainder of the England squad, including the suspended Luke Shaw, will continue their preparations at the Tottenham Hotspur training ground on Saturday.